a Record control number 000258519000012

a ISSN 1088-5412

a Original cataloging agency NEU GrandLibrary

a Language code of text/sound track or separate title eng

a Classification number WD300

b Item number .T73 2008

a Personal name Akkoç, Tunç.

h Medium Electronic Document

a Title Transfer of T cells from intranasal ovalbumin-immunized mice ameliorates allergic response in ova-sensitized recipient mice.

c Statement of responsibility, etc Tunc Akkoc, Aarif O.Eifan, Metin Aydogan, Selvinaz Ozkara, Nerin N. Bahceciler, Isil B.Barlan.

b Name of publisher, distributor, etc Ocean Side Publications Inc.,

c Date of publication, distribution, etc 2008.

a Summary, etc Mucosal immunotherapy is suggested as a treatment strategy for tolerance induction in allergic diseases. The purpose of this study was to determine the effect of transferred splenic T cells from intranasal ovalbumin (OVA)-immunized mice to naive mice before sensitization on its impact of cytokine production and airway histopathology. BALB/c mice in group I received intranasal immunotherapy (days1-6), carboxylfluorescein succinyl ester (CFSE)-labeled splenocytes or splenic T cells were i.v. transferred to naive recipients (group 11) before OVA sensitization. Acute murine asthma model was established by two i.p. OVA injections (days 21 and 28) and seven OVA nebulizations (days 42-48) in groups 1, 11 and III. Groups If[ and IV served as asthma model and control, respectively. CFSE-labeled cells in splenocytes and lymph node lymphocytes, lung histopathology, IL-4, IL-10, and interferon (IFN) gamma cytokines of recipients were analyzed 24 hours after OVA nebulization challenge. CFSE-labeled T cells from group I were detected in spleen and regional lymph nodes of the OVA-sensitized recipients (group 11). Smooth muscle and thickness of airways were less in intranasal OVA immunotherapy and OVA-sensitized recipients when compared with the asthma model (p < 0.05). Area of inflammation was significantly suppressed in OVA-sensitized recipients compared with the asthma model (p < 0.01). IL-10 and IFN-gamma levels in splenocyte supernatants were significantly increased in intranasal immunotherapy and OVA-sensitized recipients compared with asthma model and controls (P < 0.01). IL-4 levels were significantly less in intranasal immunotherapy group and the OVA-sensitized recipient group when compared with asthma the model group (p < 0.05). This study suggests that intranasal immunotherapy with allergens regulates T-cell responses and ameliorates airway histopathology in sensitized mice, hence, encouraging mucosal tolerance induction as a suitable treatment of allergic diseases.

a Topical term or geographic name as entry element Near East University Article

a Topical term or geographic name as entry element Yakın Doğu Üniversitesi Makale

a Topical term or geographic name as entry element Allergy

a Topical term or geographic name as entry element IFN-gamma

a Topical term or geographic name as entry element Intranasal immunotherapy

g Relationship information JUL-AUG 2008, Vol. 29 Issue 4, p411-416

t Title Allergy And Asthma Proceedings

x International Standard Serial Number 10885412

u Uniform Resource Identifier http://library.neu.edu.tr:2048/login?url=http://dx.doi.org/10.2500/aap.2008.29.3106